Aptagen, LLC
R12 (ID# 8217)
RNA
Bovine PrP; beta Bovine PrP
Protein
8.5 for bPrP and; 280 for its isoforms nM (reported value)
20 mM Tris-HCl (pH 7.5) and 100 mM NaCl
Selection at room temperature°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
N/A
N/A
5'-rGprGprGprGprAprGprGprAprGprGprAprGprGprAp-3'
Tsukasa Mashima, Akimasa Matsugami, Fumiko Nishikawa, Satoshi Nishikawa, Masato Katahira, Unique quadruplex structure and interaction of an RNA aptamer against bovine prion protein, Nucleic Acids Research, Volume 37, Issue 18, 1 October 2009, Pages 6249–6258, https://doi.org/10.1093/nar/gkp647
12
3824.5 g/mole
135500 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Tsukasa Mashima, Akimasa Matsugami, Fumiko Nishikawa, Satoshi Nishikawa, Masato Katahira, Unique quadruplex structure and interaction of an RNA aptamer against bovine prion protein, Nucleic Acids Research, Volume 37, Issue 18, 1 October 2009, Pages 6249–6258, https://doi.org/10.1093/nar/gkp647
Murakami, K., Nishikawa, F., Noda, K., Yokoyama, T., & Nishikawa, S. (2008). Anti-bovine prion protein RNA aptamer containing tandem GGA repeat interacts both with recombinant bovine prion protein and its β isoform with high affinity. Prion, 2(2), 73–80. https://doi.org/10.4161/pri.2.2.7024
Have your aptamer oligo synthesized ORDER
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