Aptamer Details

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Biotin (ID# 7553)

Small Organic
5.7 µM (reported value)
Selection Buffer (0.1 M KCl, 5 mM MgCl2, and 10 mM Na-HEPES (pH 7.4))
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wilson et al. "Functional Requirements for Specific Ligand Recognition by a Biotin-Binding RNA Pseudoknot." Journal of Biochemistry, 37(1998): 14410-14419.

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