Aptamer Details

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MUC1 Tumor Marker (MUC1-5TR-1) (ID# 7810)

Aptamer Chemistry: DNA

Target: MUC1 recombinant protein with five repeats of the variable tandem repeat region

Antigen/Target Category: Protein

Affinity (Kd): 47.3 nM (reported value)

Binding Conditions/Buffer: Running Buffer for SPR/Binding Buffer: 100 mM NaCl, 5 mM MgCl₂, pH 7.4.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 72

Molecular Weight: 22353.64

Extinction Coefficient: 748000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

C Ferreira et al. DNA aptamers against the MUC1 tumour marker: design of aptamer-antibody sandwich ELISA for the early diagnosis of epithelial tumors. Anal. Bioanal. Chem. 390(2008):1039-1050.

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