Aptamer Details

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a-Synuclein Oligomers (T-SO508) (ID# 7728)

Aptamer Chemistry: DNA

Target: a-synuclein oligomers

Antigen/Target Category: Protein

Affinity (Kd): 68 nM (reported value)

Binding Conditions/Buffer: TBS-T buffer for ELONA assay: 10 mM Tris-HCl, 150 mM NaCl, 5 mM KCl, pH 7.4 with 0.05% Tween 20.

Binding Temp: 25°C

Refolding Protocol: Heated to 95°C for 10 min, then cooled at a rate of 2°C/min to a final temperature of 25°C.

Specificity: Cross-reactivity with AD-specific amyloid ? oligomers (Kd=25 nM).

Does not bind to a-synuclein monomers or fibrils or PQQGDH, C-reactive protein, or luciferase. Weakly binds to IgG.

Comments: CD spectrum suggests that this aptamer forms a G-quadruplex.

Sequence ():

Length: 24

Molecular Weight: 7528.9

Extinction Coefficient: 220200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

K Tsukakoshi. Selection of DNA aptamers that recognize a-synuclein oligomers using a competitive screening method. Anal Chem. 84(2012): 5542-5547

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