Aptamer Details

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Aptamer Beacon (ID# 8174)

Aptamer Chemistry: DNA

Target: adenosine

Antigen/Target Category: Protein

Affinity (Kd): 6.0 nM (reported value)

Binding Conditions/Buffer: 150 mmol L−1 NaCl, 1 mmol L−1 MgCl2, 5 mmol L−1 KCl and Tris buffer of 20 mmol L−1 (pH 7.4)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 27

Molecular Weight: 8459.31

Extinction Coefficient: 279432

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zhang JQ, Wang YS, Xue JH, He Y, Yang HX, Liang J, Shi LF, Xiao XL. A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-"turning on" mechanism. J Pharm Biomed Anal. 2012 Nov;70:362-8. doi: 10.1016/j.jpba.2012.05.032. Epub 2012 Jun 1. PMID: 22717140.

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