Binding Buffer (0.2 M NaHCO3 and 0.5 M NaCl (pH 7.4))
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Kd values given in Table 2 of reference article may have been miscalculated. Using the kd and ka given in Table 2 of the reference, we have calculated the above Kd value from Kd=(kd/ka).