Roxithromycin (Ap01) Aptamer Details

Roxithromycin (Ap01) (ID# 10051)

Aptamer Chemistry: DNA

Target: Roxithromycin

Antigen/Target Category: Small Organic

Affinity (Kd): 0.46 ± 0.08 µM (reported value)

Binding Conditions/Buffer: Dulbecco’s Phosphate-Buffered Saline (DPBS) (0.9 mM CaCl2, 2.7 mM KCl, 1.1 mM KH2PO4, 0.5 mM MgCl2·6H2O, 0.137 M NaCl, 8.1 mM Na2HPO4, pH 7.4)

Binding Temp: 25°C

Refolding Protocol: 95 °C for 10 min; then decreased to 60 °C at a rate of 1 °C/10 s and maintained for 1 min, then continued to decrease to 25 °C at a rate of 1 °C/10 s

Specificity: Specifically binds to roxithromycin over molecules with similar structure of macrolide antibiotics and non-macrolide small molecules

Sequence ():

Length: 80

Molecular Weight: 24386.8 g/mole

Extinction Coefficient: 730200 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lingli Jiang, Min Wang, Yi Zhang, Haihua Chen, Yi Su, Yiyi Wang, Jun Sheng Lin, Preparation and characterization of DNA aptamers against roxithromycin, Analytica Chimica Acta, Volume 1164, 2021, 338509, ISSN 0003-2670, https://doi.org/10.1016/j.aca.2021.338509.

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