Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
L-Apt.4–1c (ID# 9743)
L-RNA
APP 3′-UTR D-rG4
Other
0.4 ± 0.1 nM (reported value)
25 mM Tris-HCl (pH 7.5), 1 mM MgCl2, 150 mM KCl
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5' end has a hexynyl group which is not reflected in the molecular weight or extinction coefficient. Forms an L-aptamer-antisense oligonucleotide in combination with ASO15nt(APP). L-Apt.4-1c-ASO15nt(APP) can inhibit APP protein expression.
5'-LrGLrCLrCLrCLrULrALrALrALrGLrGLrULrGLrGLrULrGLrGLrULrGLrGLrGLrALrGLrGLrGLrC-3'
25
7788 g/mole
239400 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhao, H., Lau, H. L., Zhang, K., & Kwok, C. K. (2024). Selective recognition of RNA G-quadruplex in vitro and in cells by L-aptamer-D-oligonucleotide conjugate. Nucleic acids research, 52(22), 13544–13560. https://doi.org/10.1093/nar/gkae1034 L-RNA Aptamer-Based Tools for G-Quadruplex Structure: Identification, Characterization, and Application Danyang Ji, Kun Zhang, Maryana Yarshova, and Chun Kit Kwok Accounts of Chemical Research 2025 58 (21), 3247-3258 DOI: 10.1021/acs.accounts.5c00468
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