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Apta-Index™: 1G8-14 (ID# 595)

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1G8-14 (ID# 595)

1G8-14
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Ebola Virus VP35 Protein 3.7 nM (reported value) 10 mM HEPES (pH 7.0), 150 mM NaCl, 2mM TCEP (tris(2-carboxyethyl)phosphine), and 1 mM MgCl2 and filtered through nitrocellulose membrane N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
rGprGprGprAprGprAprCprAprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprGprGprCprAprUprUprUprCprUprGprCprUprAprGprUprCprUprGprGprUprUprGprUprAprAprGprAprUprAprUprUprCprAprAprCprAprCprGprUprGprAprGprUprUprUprCprGprAprCprAprGprGprAprGprGprCprUprCprAprCprAprAprCprAprGprGprCp
90 29057.60 g/mole 914300.00 L/(mole·cm) 46.67% 1.09 31.78

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Binning, J., Wang, T., Luthra, P., et al. (2013) Development of RNA aptamers targeting Ebola Virus VP35. Biochemistry, 52(47):8406-8419. doi: 10.1021/bi400704d

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