Aptagen, LLC
RNA
Ebola Virus VP35
Protein
3.7 nM (reported value)
10 mM HEPES (pH 7.0), 150 mM NaCl, 2mM TCEP (tris(2-carboxyethyl)phosphine), and 1 mM MgCl2 and filtered through nitrocellulose membrane
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
1G8-14 (ID# 595)
270
29057.6 g/mole
914300 L/(mole·cm)
46.67%
1.09
31.78
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Binning, J., Wang, T., Luthra, P., et al. (2013) Development of RNA aptamers targeting Ebola Virus VP35. Biochemistry, 52(47):8406-8419. doi: 10.1021/bi400704d
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