Aptagen, LLC
DNA
Crude Extracellular Mixture (CEM) of E. coli
Protein
N/A nM (reported value)
Selection Buffer (50 mm HEPES (pH 7.5), 150 mM NaCl, 15 mM MgCl2, and 0.01% Tween 20), r.t.
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
In the presence of E. coli CEM, aptamer adopts an active conformation and cleaves the adenosine ribonucleotide residue, separating fluorescein from the dabcyl quencher.
nnn in structure represents (fluorescein-dT)-adenosine ribonucleotide - (dabcyl-dT).
Crude Extracellular Mixture of E. coli - cis (ID# 256)
nnn in structure represents (fluorescein-dT)-adenosine ribonucleotide - (dabcyl-dT).
5'dCpdApdCpdGpdGpdApdTpdCpdCpdTpdGpdApdCpdApdApdGpdGpdApdTpdGpdTpdGpdTpdGpdCpdGpdTpdTpdGpdTpdCpdGpdApdGpdApdCpdCpdTpdGpdCpdGpdApdCpdCpdGpdGpdApdApdCpdApdCpdTpdApdCpdApdCpdTpdGpdTpdGpdTpdGpdGpdGpdApdTpdGpdGpdApdTpdTpdTpdCpdTpdTpdTpdApdCpdApdGpdTpdTpdGpdTpdGpdTpdGpdCpdApdGpdCpdTpdCpdCpdGpdTpdCpdCpdGpdApdCpdTpdCpdTpdTpdCpdCpdTpdApdGpdCp{Internal/Fluorescein-dT}rAp{Internal/Dabcyl-dT}dGpdGpdTpdTpdCpdGpdApdTpdCpdApdApdGpdAp3'
111
38623.03 g/mole
1183600 L/(mole·cm)
52.80%
0.84
32.63
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ali et al. "Fluorogenic DNAzyme Probes as Bacterial Indicators." Angewandte Chemie International Edition, 50 (2011): 3751-3754.
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