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Apta-Index™: L-Isoleucine (IL 42-32b) (ID# 112)

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L-Isoleucine (IL 42-32b) (ID# 112)

L-Isoleucine (IL 42-32b)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA L-Isoleucine Small Organic 200 nM (reported value) Selection Buffer (10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl) Reaction Buffer (50 mM sodium borate (pH 8.0), 42 mM KCl, 7.5 mM MgCl2, and 0.4 mM ZnCl2) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-P-rGprGprUprCprUprUprAprCprGprUprCprGprUprUprCprGprCprGprAprCprUprAprUprUprGprGprGprAprGprAprCprCp-3'
32 10233.16 g/mole 310800.00 L/(mole·cm) 56.25% 3.22 32.93

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Majerfeld and Yarus. "Isoleucine:RNA sites with associated coding sequence." RNA, 4 (1998): 471-478.

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