Aptagen, LLC
TAR S3 (ID# 9283)
RNA
Intranuclear RNA-protein interactions involving HIV-1 Tat protein and TAR RNA
Protein
<1.0 pM (reported value)
Buffer: 50 mM Tris, 50 mM KCl, 0.01% Triton-X-100, pH = 7.4.
25 °C °C
Heated to 95°C for 5 minutes, rapidly cooled on ice for 5 minutes, and then incubated at 25°C for 10 minutes. 200 µL of SELEX buffer (containing 10 mM MgCl2, 50 mM NaCl, 0.1% Tween 20, 0.1 mg/ml tRNA, and 25 mM Tris, pH 7.6).
The U's in the sequence represent indole modified T bases.
5'-rCprAprCprGprAprCprGprCprAprAprGprGprGprAprCprCprAprCprAprGprGprAprGprGprCprCprCprAprGprGprCprUprGprCprUprUprGprCprCprCprCprGprGprAprAprCprCprAprGprCprAprCprGprAprCprAprCprCprGprCprAprGprAprGprGprCprAp-3'
67
20589.3 g/mole
635700 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Li, J., Yao, P., Tang, K., Zhao, X., Liu, X., Liu, Q., Wei, T., Xuan, H., Bian, S., Guo, Y., Yang, Z., Zhang, Z. Q., & Zhang, L. (2024). Functional Aptamers In Vitro Evolution for Intranuclear Blockage of RNA-Protein Interaction. Journal of the American Chemical Society, 146(35), 24654–24662. https://doi.org/10.1021/jacs.4c08824.
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