Aptamer Details

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R7-02 ssDNA aptamer scaffold for SARS-CoV-2 RBD (HOLD-selected) (ID# 9703)

Aptamer Chemistry: DNA

Target: SARS-CoV-2 Spike RBD

Antigen/Target Category: Protein

Affinity (Kd): 5.702 nM (reported value)

Binding Conditions/Buffer: 1X PBSMT [consisted of 1× PBS (pH 7.4), 2.5 mM MgCl2 and 0.02% Tween 20]

Binding Temp: 25°C

Refolding Protocol: heat at 95 °C for 5 minutes, snap-cooled in ice, room temperature for 1 hour

Specificity: Binds to SARS-CoV-2 Spike RBD (wild type and multiple variants, including Omicron)

Comments: 3' FAM and 5' Hexynyl modifications on R7-02. HOLD-selected refers to the selection method used to generate the R7-02 aptamer scaffold–peptide hybrid, and it’s a specialized variant of aptamer selection.

Sequence ():

Length: 60

Molecular Weight: 18789.2 g/mole

Extinction Coefficient: 603800 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lee, M., Kang, B., Lee, J., Lee, J., Jung, S. T., Son, C. Y., & Oh, S. S. (2022). De novo selected hACE2 mimics that integrate hotspot peptides with aptameric scaffolds for binding tolerance of SARS-CoV-2 variants. Science advances, 8(43), eabq6207. https://doi.org/10.1126/sciadv.abq6207

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