Aptagen, LLC
Oxytetracycline binding aptamer (OTC-4) (ID# 8002)
DNA
Oxytetracycline
Small Organic
9.61 nM (reported value)
100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM
CaCl2, 0.02 Tween 20, pH 7.6
25°C
90 C for 10 min, quickly cooled at 4 C for 15 min and incubated at 25 C for 7 min.
Highly specific for oxytetracycline over other tetracycline derivatives.
High affinity for small target partially explained by intrinsic oligonucleotide binding properties of tetracycline derivatives.
5'-dCpdGpdTpdApdCpdGpdGpdApdApdTpdTpdCpdGpdCpdTpdApdGpdCpdCpdGpdApdCpdGpdCpdGpdCpdGpdTpdTpdGpdGpdTpdGpdGpdTpdGpdGpdApdTpdGpdGpdTpdGpdTpdGpdTpdTpdApdCpdApdCpdGpdTpdGpdTpdTpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdCpdApdCpdGpdTpdGp-3'
76
23601.29
791330
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Niazi, J. H., Lee, S. J., Kim, Y. S., & Gu, M. B. (2008). ssDNA aptamers that selectively bind oxytetracycline. Bioorganic & Medicinal Chemistry, 16(3), 1254–1261. doi:10.1016/j.bmc.2007.10.073
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