Aptamer Details

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M5 (ID# 9590)

Aptamer Chemistry: DNA

Target: Mutant Q78 Huntigtin

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Binding conditions were evaluated with a typical ELISA. Binding depletion type of ELISA was conducted to evaluate aptamer binding efficiency.

Binding Temp: 25°C

Refolding Protocol: N/A

Specificity: Upon digestion of biotinylated aptamer-mutant huntingtin complex with caspase 6, the aptamer specifically bound to the C terminal region of the protein. Aptamer specific to Q78 huntingtin, not Q23 huntingtin.

Comments: Among the four aptamers tested, MS3 interacted better with Q78 huntingtin and selectively reduced its PRC2 stimulating activity.

Sequence ():

Length: 36

Molecular Weight: 11645.5 g/mole

Extinction Coefficient: 393400 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shin, B., Jung, R., Oh, H., Owens, G. E., Lee, H., Kwak, S., Lee, R., Cotman, S. L., Lee, J. M., MacDonald, M. E., Song, J. J., Vijayvargia, R., & Seong, I. S. (2018). Novel DNA Aptamers that Bind to Mutant Huntingtin and Modify Its Activity. Molecular therapy. Nucleic acids, 11, 416–428. https://doi.org/10.1016/j.omtn.2018.03.008

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