Aptamer Details

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L1 (ID# 9678)

Aptamer Chemistry: DNA

Target: IGFBP3 (Insulin-like Growth Factor Binding Protein 3)

Antigen/Target Category: Protein

Affinity (Kd): 8 nM (reported value)

Binding Conditions/Buffer: Library was injected into ovarian bursa of female C57BL6/athymic nude mice for in vivo SELEX. 4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL BSA, 0.1 mg/mL salmon sperm DNA in DPBS, pH 7.4 at 37 °C for 30 min in the dark.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: In vivo SELEX with a DNA pool enriched through prior in vitro cell-SELEX on ovarian cancer patient-derived cells (ID8/PDC3). Mice were intravenously injected with 200 pmol of Cy5-labeled pool and sacrificed 1 h later for tumor collection. 3 pools/rounds were done to obtain current sequence.

Sequence ():

Length: 53

Molecular Weight: 16122.4 g/mole

Extinction Coefficient: 475500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Li, C., Chen, W., Zhang, Z., Qiu, F., Jiang, R., Fang, X., Zhang, S., Li, L., Liao, G., Shen, Y., Tang, H., Meng, H., Zhu, T., Luo, Z., & Tan, W. (2025). In Vivo SELEX: A Strategy for Identifying Ovarian Cancer-Specific Aptamers. ACS applied materials & interfaces, 17(46), 63162–63171. https://doi.org/10.1021/acsami.5c17714

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