Aptamer Details

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iSN04 Nucleolin Aptamer (ID# 8957)

Aptamer Chemistry: DNA

Target: Nucleolin

Antigen/Target Category: Protein

Binding Conditions/Buffer: Internalized to cells grown in DMEM, 15% FBS, 1% non-essential amino acids (NEAA) (Fujifilm Wako Chemicals), 1% nucleosides (Merck, Darmstadt, Germany), 0.1 mM 2-mercaptoethanol (Nacalai), 103 units/mL LIF (Nacalai), 3 uM CHIR99021 (FujifilmWako Chemicals), 0.8 uM PD0325901 (FujifilmWako Chemicals), and P/S

Binding Temp: 37°C

Refolding Protocol: N/A

Comments: Binding affinity not determined in Ishioka et al., 2023. Authors state that "[the aptamer] forms a guanine quadruplex-like structure".

Sequence ():

Length: 18

Molecular Weight: 5956.8

Extinction Coefficient: 193500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ishioka M, Nihashi Y, Sunagawa Y, et al. Myogenetic Oligodeoxynucleotide Induces Myocardial Differentiation of Murine Pluripotent Stem Cells. Int J Mol Sci. 2023;24(18):14380. Published 2023 Sep 21. doi:10.3390/ijms241814380

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