Aptamer Details

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Insulin Binding Aptamer (IBA) (ID# 7960)

Aptamer Chemistry: DNA

Target: Insulin

Antigen/Target Category: Protein

Affinity (Kd): n/a nM (reported value)

Binding Conditions/Buffer: 50 mM Tris-HCl, 10 mM KCl, 100 mM NaCl

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specificity tested against Streptavidin, Avidin, and BSA.

Comments: FAM-Labeled at 3' end.

Sequence ():

Length: 30

Molecular Weight: 9493.19

Extinction Coefficient: 288200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Pu, Y.; Zhu, Z.; Han, D.; Liu, H.; Liu, J.; Liao, J.; Zhang, K.; & Tan, W. Insulin-Binding Aptamer-Conjugated Graphene Oxide for Insulin Detection. Analyst, 2011, 136, 4138-4140

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