Aptamer Details

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IFNγ Aptamer (ID# 9256)

Aptamer Chemistry: DNA

Target: Interferon-gamma (IFNγ)

Antigen/Target Category: Protein

Affinity (Kd): ~10 nM (reported value)

Binding Conditions/Buffer: The buffer is RPMI with 10% FBS, incubated at 37 °C (handled at room temperature). It uses 1 nmol per 10⁶ cells to detect IFNγ within 48 hours, anchored by 3′-CholTEG membrane insertion.

Binding Temp: Room temperature (22–25 )°C

Refolding Protocol: Resuspend at 10 µM in nuclease-free water, heat to 95 °C for 5 min, snap-cool on ice for 5 min, equilibrate at room temp for 30 min, then add to cells (1–2 nmol/10⁶ cells).

Specificity: Highly specific to human IFNγ, with no response to unrelated antigens or IFNG-knockout T cells. No signal with non-target aAPCs.

Comments: Molecular beacon aptamer for cytokine-based TCR screening; flow cytometry-compatible with reversible stem-loop signaling.

Sequence ():

Length: 36

Molecular Weight: Approx. 10,700 – 11,000 Da

Extinction Coefficient: 354,000 M⁻¹cm⁻¹

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Luo, S., Notaro, A., & Lin, L. (2025). ATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs. Nature Communications, 16, 216. https://doi.org/10.1038/s41467-024-54675-3

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