Aptamer Details

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HuNoV-GII Aptamer (ID# 9298)

Aptamer Chemistry: DNA

Target: Human Norovirus GII.4 & GII.17 Virus-Like Particles (VLPs)

Antigen/Target Category: Protein

Affinity (Kd): 38.63 (AP4-2), 4.67 (AP17-4) nM (reported value)

Binding Conditions/Buffer: Binding assays were conducted at 37°C using PBST buffer supplemented with 5 mM MgCl₂. Aptamer concentrations ranged from 0.01 to 2.0 µM, and target VLP concentrations ranged from 1.5 to 10 µg/mL.

Binding Temp: 37°C

Refolding Protocol: The aptamers were refolded by heating at 95°C for 5 minutes, followed by slow cooling to room temperature in binding buffer containing 5 mM MgCl₂ for optimal target binding.

Specificity: The aptamers AP4-2 and AP17-4 exhibited high specificity for HuNoV GII.4 and GII.17 VLPs, respectively, with no significant cross-reactivity to unrelated pathogens or proteins. Specificity was validated by dot blotting and ELASA, showing strong target binding and minimal background signal (P < 0.0001).

Sequence ():

Length: 79 Nucleotides

Molecular Weight: ~18-25 kDa

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Cheng, C., Sun, M., Li, J., Xue, Y., Cai, X., Liu, J., Wang, X., Xu, S., Xie, Y., & Zhang, J. (2025). Nucleic acid aptamers for human norovirus GII.4 and GII.17 virus-like particles (VLPs) exhibit specific binding and inhibit VLPs from entering cells. International Journal of Nanomedicine, 20, 1789–1805. https://doi.org/10.2147/IJN.S495399

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