Aptagen, LLC
H Seq02 against Sterigmatocystin (ID# 9187)
DNA
Sterigmatocystin (ST) - water-insoluble small-molecule targe
Small Organic
25.3 nM (reported value)
Molecular Activation of ST: Glycolic acid (C2H4O3, 1 g) dissolved in 4 mL of trifluoroacetic acid (C2HF3O2), 4 mL of 2.5 mg/mL ST.
Capture SELEX: 200ul of Dulbecco’s phosphate-buffered saline (DPBS), pH of 7.4
Aptamer Affinity Column: 1 mL of blocking solution (0.2% glycine solution, pH of 6.0), 1 mL of DPBS, 1 mL of PBST, and 1 mL of water.
25 °C°C
Library and Biotin primer were mixed at a1:2 ratio and treated at 95 °C for 10 min, 60 °C for 1 min, and 25 °C for 1 min. Further, it was mixed with washed magnetic beads and incubated for 80 min at room temperature in a rotary shaker.
Based on Aptamer Affinity Column using 2.3 nmol of H Seq02, showed a binding capacity of 80 ng.
Circular dichroism and isothermal titration calorimetry further confirmed the aptamer's interaction with the target ST, supporting its potential for developing new ST detection methods.
5'-dGpdApdApdTpdApdCpdGpdGpdApdTpdTpdApdCpdTpdGpdTpdTpdCpdGpdTpdApdCpdTpdGpdApdCpdCpdTpdApdTpdGpdCpdTpdGpdTpdGpdGpdApdGpdTp-3'
40
12357 g/mole
387200 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zheng, M., Ye, J., Liu, H., Wu, Y., Shi, Y., Xie, Y., & Wang, S. (2024). FAM Tag Size Separation-Based Capture-Systematic Evolution of Ligands by Exponential Enrichment for Sterigmatocystin-Binding Aptamers with High Specificity. Analytical chemistry, 96(2), 710–720. https://doi.org/10.1021/acs.analchem.3c03675.
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