Aptagen, LLC
Fezf2 Binding Site (Fragment 4.26) (ID# 10210)
DNA
Forebrain embryonic zinc finger 2 (Fezf2)
Protein
29 nM (reported value)
20 mM HEPES at pH7.9, 8% glycerol, 10 mM MgCl2, 100 mM KCl, 10 uM Zn(C2H3O2)2
Room temperature°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specifically binds to the wild type sequence over the mutated sequence.
The sequence is a double stranded DNA binding site for Fezf2, identified through genomic SELEX.
5'-dCpdCpdCpdCpdCpdTpdCpdCpdTpdTpdCpdTpdGpdCpdGpdApdCpdApdCpdGpdCpdTpdTpdCpdGpdCpdTpdTpdTpdTpdTpdGpdApdTpdGpdCpdCpdTpdGpdCpdTpdCpdCpdTpdGpdApdGpdTpdGpdTpdCpdApdGpdCpdApdApdCpdCpdCpdTpdCpdTpdCpdTpdTpdTpdCpdApdGpdTpdCpdTpdCpdCpdTpdTpdCpdTpdGpdTpdCpdTpdGpdTpdCpdTpdGpdTpdCpdApdGpdApdCpdGp-3'
94
28522.4 g/mole
809600 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
L. Chen, J. Zheng, N. Yang, H. Li, S. Guo. (2011) Genomic selection identifies vertebrate transcription factor Fezf2 binding sites and target genes. Biological Chemistry, 296(21), 18641-18649. DOI 10.1074/jbc.M111.236471.
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