Aptamer Details

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Es2-7bp (17β-estradiol) (ID# 9522)

Aptamer Chemistry: DNA

Target: 17β-estradiol

Antigen/Target Category: Small Organic

Affinity (Kd): 0.6 µM (reported value)

Binding Conditions/Buffer: 20 mM HEPES pH 7.4, 100 mM NaCl, 8 mM MgCl2

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Aptamer switch

Sequence ():

Length: 43

Molecular Weight: 13317.7 g/mole

Extinction Coefficient: 426500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shi, L., Jin, Y., & Liu, J. (2024). Intramolecular aptamer switches. The Analyst, 149(3), 745–750. https://doi.org/10.1039/d3an02022c

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