Aptamer Details

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Enro_Ap3 (Enrofloxacin) (ID# 9250)

Aptamer Chemistry: DNA

Target: Enrofloxacin

Antigen/Target Category: Small Organic

Affinity (Kd): 1.64 ± 0.181 µM (reported value)

Binding Conditions/Buffer: In SELEX Buffer (see Refolding Protocol) at 25°C with shaking at 200 rpm.

Binding Temp: 25°C

Refolding Protocol: Hybridized in SELEX buffer (20 mM NaCl, 10 mM MgCl2, 6mM KCl, 5 mM CaCl2, 4% DMSO, and 40 mM Tris-HCL at pH 7.5) for 5 minutes at 95°C, cooled to 25°C for 10 minutes and left overnight at 4°C.

Sequence ():

Length: 30

Molecular Weight: 9309.2 g/mole

Extinction Coefficient: 321900 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Enhanced Detection of Enrofloxacin in Seawater Using a Newly Selected Aptamer on a Graphite Oxide-Based Biosensor Sin Yu Lam, Pan Li, Linjie Jin, Hau Yi Chan, Yuefei Ruan, Chun Kit Kwok, and Pik Kwan Lo Analytical Chemistry 2025 97 (12), 6735-6744 DOI: 10.1021/acs.analchem.4c07052

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