Aptamer Details

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Aptamer Apt1 Against Spike (S) Protein of SARS-CoV-2 (ID# 9638)

Aptamer Chemistry: DNA

Target: Spike Protein (S -Protein) of SARS-CoV-2

Antigen/Target Category: Protein

Affinity (Kd): 10.2 ± 2.7 nM (reported value)

Binding Conditions/Buffer: Phosphate-buffered saline (PBS; 10 mM phosphate buffer, 137 mM NaCl, 2.7 mM KCl, pH 7.4), Aptamer Binding Buffer was PBS containing 0.55 mM MgCl2.

Binding Temp: 25°C

Refolding Protocol: N/A

Specificity: The aptamer sandwich assay specifically detected the SARS-CoV-2 spike protein at ~21 ng/mL (270pM) with negligible cross-reactivity against spike proteins from other human coronaviruses.

Sequence ():

Length: 76

Molecular Weight: 23587.2 g/mole

Extinction Coefficient: 724900 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Svobodova, M., Skouridou, V., Jauset-Rubio, M., Viéitez, I., Fernández-Villar, A., Cabrera Alvargonzalez, J. J., Poveda, E., Bofill, C. B., Sans, T., Bashammakh, A., Alyoubi, A. O., & O'Sullivan, C. K. (2021). Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen. ACS omega, 6(51), 35657–35666. https://doi.org/10.1021/acsomega.1c05521

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