Aptagen, LLC
Ap 25 (ID# 9375)
DNA
Omicron SARS-COV-2 Nucleocapsid
Small Organic
Aptamers (200 nM concentration in folding buffer) are heated to 95 degrees Celsius for 5 minutes and placed on ice. Nucleocapsid proteins are incubated with 100 ul of aptamers at a 200 nM concentration for 2 hours and washed with 300 ul of 1x ELISA wash buffer.
37 °C°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specifically binds better to the Omicron variant of SARS-COV-2.
CGA GGC TCT CGG GAC GAC ATC TTC AGC GAT TCT TAA ACC TTA GAG TAT GTC GTC CCG CCT TTA GGA TTT ACA dGp
73
22428.5 g/mole
689900 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Charles P. Neff, Mile Cikara, Brian J. Geiss, G. Thomas Caltagirone, Albert Liao, Shaikh M. Atif, Bradley Macdonald, Richard Schaden. Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2, Current Research in Biotechnology, Volume 5. 2023. 100132. ISSN 2590-2628. https://doi.org/10.1016/j.crbiot.2023.100132.
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