Aptamer Details

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Ap 2 (ID# 9373)

Aptamer Chemistry: DNA

Target: SARS-COV-2 Nucleocapsid

Antigen/Target Category: Small Organic

Binding Conditions/Buffer: Aptamers are heated to 95 degrees Celsius for 5 minutes and placed on ice. The proteins are then incubated 100 ul of aptamers at a 200 nM concentration for 2 hours and then washed with 300 ul of 1x ELISA buffer.

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specifically binds better to the alpha variant of SARS-COV-2.

Sequence ():

Length: 73

Molecular Weight: 22478.6 g/mole

Extinction Coefficient: 691500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Charles P. Neff, Mile Cikara, Brian J. Geiss, G. Thomas Caltagirone, Albert Liao, Shaikh M. Atif, Bradley Macdonald, Richard Schaden. Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2, Current Research in Biotechnology, Volume 5. 2023. 100132. ISSN 2590-2628. https://doi.org/10.1016/j.crbiot.2023.100132.

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