Aptamer Details

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ProApt-15 (ID# 9308)

Aptamer Chemistry: DNA

Target: Procymidone (a dicarboximide fungicide)

Antigen/Target Category: Small Organic

Affinity (Kd): 24.22 nM (reported value)

Binding Conditions/Buffer: Buffer: 1×BB buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl₂, pH 7.4) Incubation: 1 hr at RT with target; 2 h GO incubation for fluorescence assay Denaturation/Refolding: 95 °C for 10 min → 60 °C for 1 min → 4 °C for 5 min (cooling at 0.1 °C/s)

Binding Temp: 25°C

Refolding Protocol: The aptamer was refolded by heating at 95 °C for 10 minutes, followed by cooling to 60 °C for 1 minute, then rapidly cooling to 4 °C for 5 minutes before use.

Specificity: Specific for procymidone; no cross-reactivity to iprodione, chlozolinate, dimethachlon, or vinclozolin

Comments: Seq15 was developed via Capture-SELEX using an immobilized ssDNA library and showed high specificity and sensitivity. It was used in a lateral flow assay with a detection limit of 0.79 ng/mL in spiked vegetable samples.

Sequence ():

Length: 74 nucleotides

Molecular Weight: ~23.8 kDa

Extinction Coefficient: ~750,000 M⁻¹cm⁻¹

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Dong, H., Wang, H., Guo, Z., Huang, J., Zhang, P., Guo, Y., & Sun, X. (2024). Combination of Capture-SELEX and Post-SELEX for procymidone-specific aptamer selection and broad-specificity aptamer discovery, and development of aptamer-based lateral flow assay. Analytica Chimica Acta, 1318, 342922. https://doi.org/10.1016/j.aca.2024.342922

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