Aptagen, LLC
71 (ID# 9591)
DNA
BACE1
Protein
12.0±1.2 nM (reported value)
1 mL of selection buffer (137 mM NaCl, 2.7 mM KCl, 6. 5 mM Na₂HPO₄, 1.8 mM NaH₂PO₄,, 1.5 mM MgCl₂, and 0.1% (w/v) BSA, pH 7.4)
25°C
Heated at 75°C for 3 min in selection buffer and then slowly cooled to 37°C for at least 10 minutes. Then incubated in rotating wheel for 30 minutes to room temperature.
T should be replaced with X=5-CIU from aptamer 71T from nucleotide 21 to 67 and that would be sequence .
5'-dGpdCpdCpdTpdGpdTpdTpdGpdTpdGpdApdGpdCpdCpdTpdCpdCpdTpdApdApdCpdGpdGpdCpdGpdTpdApdGpdGpdGpdTpdTpdTpdGpdTpdTpdGpdTpdCpdTpdCpdTpdApdTpdTpdTpdCpdTpdTpdTpdGpdTpdApdTpdTpdGpdTpdGpdTpdTpdGpdTpdGpdTpdTpdCpdGpdCpdTpdCpdApdTpdGpdCpdTpdTpdApdTpdTpdCpdTpdTpdGpdTpdCpdTpdCpdCpdCp-3'
89
27308.6 g/mole
792200 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Gasse C, M Zaarour, S Noppen, M Abramov, P Marlie`re, S Liekens, B De Strooper and P Herdewijn. (2018). Modulation of BACE1 activity by chemically modified aptamers. ChemBioChem 19:754–763. DOI: 10.1002/cbic.201700461
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