Aptamer Details
- Aptamer Chemistry
- RNA
- Target
- Yeast phenylalanine tRNA
- Antigen/Target Category
- Small Organic
- Affinity (Kd)
- 12 nM (reported value)
- Binding Conditions/Buffer
- Selection Buffer (250 mM NaCl, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM spermidine, 0.2 mM EDTA)
- Binding Temp
- 25 °C
- Refolding Protocol
-
NA
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
- Sequence ( )
-
5'--3'
- Length
- 109
- Molecular Weight
- 35011.9 g/mole
- Extinction Coefficient
- 1060400 L/(mole·cm)
- Reference
-
Scarabino, Daniela, et al. "tRNA prefers to kiss." EMBO Journal 18 (1999): 4571-4578
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Related Aptamer Targets
There are no aptamers related to this aptamer.
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