Aptamer Details
* The secondary structure may not be accurate because of
MFOLD limitations on chemistries other than RNA/DNA.
- Aptamer Chemistry
- 2'-F-RNA
- Target
- hlgG-Fc
- Antigen/Target Category
- Protein
- Affinity (Kd)
- 75 nM (reported value)
- Binding Conditions/Buffer
- Binding Buffer (145mM NaCl, 5.4 mM KCl, 0.8mM MgCl, 1.8 mM CaCl, and 20 mM Tris-HCl (pH 7.6)) Modifications at the 5' and 3' end have been made by the authors for various testing.
- Binding Temp
- N/A
- Refolding Protocol
-
NA
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
- Specificity
-
Specific for Human IgG, subclasses hIgG1 through hIgG4.
Does not bind to mouse, rat, rabbit, bovine, chicken, canine, feline, and guinea pig IgG. - Comments
-
This aptamer binds to the Fc domain of hIgG.
See also the modified version of this aptamer, 8-2. - Sequence ( )
-
5'--3'
- Length
- 23
- Molecular Weight
- 7442.54 g/mole
- Extinction Coefficient
- 226600 L/(mole·cm)
- Reference
-
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Related Aptamer Targets
There are no aptamers related to this aptamer.
We are always looking for ways to improve. Please tell us what you think.