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Apta-Index™: Sialyllactose (Sl-11) (ID# 90)

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Sialyllactose (Sl-11) (ID# 90)

Sialyllactose (Sl-11)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Sialyllactose Other 4.9 µM (reported value) Binding Buffer (50 mM Tris-HCl (pH 7.6), 250 mM NaCl, 5 mM MgCl2) Denatured at 95°C for 5 min and cool at RT for 30 min. Except for the primer 1 region, thymidines contained an amino linker with terminal amine at the C5 position. This was an attempt to offset the repulsive force between the negatively-charged target and DNA backbone.
5'-dGpdTpdApdCpdGpdApdApdTpdTpdCpdApdCpdGpdApdGpdGpdTpdTpdGpdCpdCpdApdGpdCpdGpdGpdGpdGpdCpdCpdApdGpdCpdCpdApdCpdTpdTpdCpdTpdGpdTpdCpdApdGpdTpdGpdApdApdTpdTpdCpdCpdTpdGpdCpdTpdCpdGpdTpdApdTpdApdTpdCpdTpdApdCpdTpdCpdGpdCpdCpdCpdGpdCpdCpdTpdGpdCpdGpdApdGpdCpdApdTpdGpdGpdApdGpdTpdCpdGpdGpdApdTpdCpdCpdTpdCpdTpdAp-3'
102 31347.28 g/mole 947500.00 L/(mole·cm) 56.86% 1.06 33.08

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Masud, M, et al. "Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX." Bioorganic & Medicinal Chemistry, 12 (2004): 1111-1120.

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