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Apta-Index™: Broccoli (ID# 649)

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Broccoli (ID# 649)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA (Z)-4-(3,5- difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol5(4H)-one (GFHBI) Small Organic 360 nM (reported value) In bacterial or mammalian cells, PBS buffer, etc. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Several variants have been developed for applications such as programmable RNA detection (Furuhata 2019 RNA Journal).

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution Grigory S. Filonov, Jared D. Moon, Nina Svensen, and Samie R. Jaffrey Journal of the American Chemical Society 2014 136 (46), 16299-16308 DOI: 10.1021/ja508478x

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