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Apta-Index™: Thrombin (15mer) (ID# 64)

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Thrombin (15mer) (ID# 64)

Thrombin (15mer)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Thrombin Protein 25-200 nM (reported value) Selection Buffer: (20 mM Tris-acetate pH 7.4, 40mM NaCl, 5mM KCl, 1mM CaCl2, 1mM MgCl2) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Uehara et al. (2008) found that conjugation of a poly(dA) tail to the 5' end disrupts binding to thrombin.

The 29mer version has higher affinity than the 15mer version, but less inhibitory activity.
5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGp-3'
15 4726.13 g/mole 143300.00 L/(mole·cm) 60.00% 6.98 32.98

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Bock, Louis, et al. "Selection of single-stranded DNA molecules that bind and inhibit human thrombin." Nature Journal 355, (1992): 564-566.

S Uehara et al. 30 Poly(dA)-Tailed Thrombin DNA Aptamer to Increase DNase-Resistance and Clotting Inhibitory Activity. Bull. Chem. Soc. Jpn. 81(2008): 1485–1491

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