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Apta-Index™: GR-3 (ID# 632)

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GR-3 (ID# 632)

GR-3
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA glucagon receptor (GCGR) Protein 52.7±5.1 nM (reported value) 5mM MgCl2 and 4.5 g/L glucose in Dulbecco’s Phosphate Bufered Saline (D-PBS, pH=7.4), and 0.1mg/mL yeast tRNA and 1mg/L bovine serum albumin (BSA) to reduce nonspecifc binding. denatured at 95 °C for 5 min and then cooled immediately on ice for 10 min
3'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdGpdApdTpdApdApdGpdTpdApdGpdGpdTpdApdTpdCpdCpdGpdTpdTpdTpdGpdApdApdApdApdApdCpdTpdTpdTpdTpdCpdTpdGpdApdCpdCpdGpdTpdCpdCpdGpdApdCpdTpdApdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp-5'
78 24099.71 g/mole 760100.00 L/(mole·cm) 46.91% 1.32 31.71

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wang, Guodong, et al. "Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX". Nature: Scientific Reports (7): 7179. DOI:10.1038/s41598-017-05840-w

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