Aptamer Details

Antibac1 (ID# 630)

Aptamer Chemistry: DNA Target: Peptidoglycan Antigen/Target Category: Other Affinity (Kd): 415 nM (reported value) Binding Conditions/Buffer: PBS 1x Binding Temp: 23°C Refolding Protocol: N/A Specificity: 0.415 uM for peptidoglycan 31.82 nM for E. coli Comments: This aptamer was originally selected for peptidoglycan, but it is referenced in an apta-report because it was also demonstrated to have affinity for a variety of bacteria cells, including E. coli by Ana Claudia Graziani, Maria Isabel Stets, Ana Luisa Kalb Lopes, Pedro Henrique Caires Schluga, Soledad Marton, Ieda Ferreira Mendes, Antero Silva Ribeiro de Andrade, Marco Aur??lio Krieger, Josiane Cardoso. J. Microbiol. Biotechnol.2017 ; 27(4): 838~843

Sequence (): 5'dTpdCpdGpdCpdGpdCpdGpdApdGpdTpdCpdGpdTpdCpdTpdGpdGpdGpdGpdApdCpdApdGpdGpdGpdApdGpdTpdGpdCpdGpdCpdTpdGpdCpdTpdCpdCpdCpdCpdCpdCpdGpdCpdApdTpdCpdGpdTpdCpdCpdTpdCpdCpdCp3' Length: 55 Molecular Weight: 7395.81 g/mole Extinction Coefficient: 220800 L/(mole·cm) GC Content: 72.73% nmoles/OD₂₆₀: 4.53 μg/OD₂₆₀: 33.50

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ferreira IM, de Souza Lacerda CM, de Faria LS, Corrêa CR, de Andrade AS. Selection of peptidoglycan-specific aptamers for bacterial cells identification. Appl Biochem Biotechnol. 2014 Dec;174(7):2548-56. doi: 10.1007/s12010-014-1206-6. Epub 2014 Sep 4. PMID: 25185503.

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Aptamer Details

Antibac1 (ID# 630)

Ferreira IM, de Souza Lacerda CM, de Faria LS, Corrêa CR, de Andrade AS. Selection of peptidoglycan-specific aptamers for bacterial cells identification. Appl Biochem Biotechnol. 2014 Dec;174(7):2548-56. doi: 10.1007/s12010-014-1206-6. Epub 2014 Sep 4. PMID: 25185503.

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