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Apta-Index™: CAP/CAM Aptamer (ID# 629)

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CAP/CAM Aptamer (ID# 629)

CAP/CAM Aptamer
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Chloramphenicol Other 7.7 µM (reported value) 100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. AptaReport Article Reference: Luan Q., Xi Y., et al. (2017) A facile colorimetric aptamer assay for small molecule detection in food based on a magnetic single-stranded DNA binding protein-linked composite probe. Sensors and Actuators B: Chemical 239: 979-978.
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdCpdTpdTpdCpdApdGpdTpdGpdApdGpdTpdTpdGpdTpdCpdCpdCpdApdCpdGpdGpdTpdCpdGpdGpdCpdGpdApdGpdTpdCpdGpdGpdTpdGpdGpdTpdApdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdCpdGpdTpdGpdApdAp-3'
81 25106.28 g/mole 781800.00 L/(mole·cm) 58.02% 1.28 32.11

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Mehta J, Van Dorst B, Rouah-Martin E, et al. (2010) In vitro selection and characterication of DNA aptamers recognizing chloramphenicol. Journal of Biotechnology 155: 361-369.

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