Aptagen, LLC
DNA
Interferon-gamma (IFN-c)
Protein
74.5 nM (reported value)
Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) were added to the binding buffer (PBS) to reduce the background interference
°C
The procedures of selection were as follows: in the first selection round, the ssDNA pool (200 pmol) was first heated at 95uC for 5 min and then cooled to 0uC for 15 min immediately.
To evaluate the binding specificity of aptamers to target, FITClabeled aptamer was incubated with IFN-c, paraformaldehydefixed IFN-c, trypsin, IgG or BSA coated magnetic beads, respectively. The beads were washed twice with 0.5 ml of binding buffer, suspended in 0.2 ml of binding buffer, and ana
Interferon-Gamma (ID# 619)
5'dCpdCpdGpdCpdCpdCpdApdApdApdTpdCpdCpdCpdTpdApdApdGpdApdGpdApdApdGpdApdCpdTpdGpdTpdApdApdTpdGpdApdCpdApdTpdCpdApdApdApdCpdCpdApdGpdApdCpdApdCpdApdCpdTpdApdCpdApdCpdApdCpdGpdCpdAp3'
59
18001.76 g/mole
585100 L/(mole·cm)
47.46%
1.71
30.77
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Cao, B., Hu, Y., Duan, J., Ma, J., Xu, D., & Yang, X. D. (2014). Selection of a novel DNA aptamer for assay of intracellular interferon-gamma. PloS one, 9(5), e98214.
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