* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Interferon-gamma (IFN-c) Protein 74.5 nM (reported value)Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) were
added to the binding buffer (PBS) to reduce the background
interferenceThe procedures of selection were as follows: in the
first selection round, the ssDNA pool (200 pmol) was first heated at
95uC for 5 min and then cooled to 0uC for 15 min immediately.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Cao, B., Hu, Y., Duan, J., Ma, J., Xu, D., & Yang, X. D. (2014). Selection of a novel DNA aptamer for assay of intracellular interferon-gamma. PloS one, 9(5), e98214.
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
Interferon-gamma (IFN-c)
Protein
74.5 nM (reported value)
Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) were
added to the binding buffer (PBS) to reduce the background
interference
The procedures of selection were as follows: in the
first selection round, the ssDNA pool (200 pmol) was first heated at
95uC for 5 min and then cooled to 0uC for 15 min immediately.
