Aptamer Details

Beta-Estradiol Aptamer (BES.1) (ID# 609)

Aptamer Chemistry: DNA Target: Beta-Estradiol (Estrogen) Antigen/Target Category: Small Organic Affinity (Kd): 6 µM (reported value) Binding Conditions/Buffer: 1X Binding Buffer: 20 mm Tris (pH 7.4), 1 M NaCl, 10 mM MgCl₂ Binding Temp: 23°C Refolding Protocol: 95°C for 5 minutes, allow aptamer to cool to room temperature over 10 minutes Specificity: Specific to beta-estradiol over deoxycholic acid. Shows cross-reactivity towards dehydroisoandrosterone 3-sulfate and deoxycorticosterone 21-glucoside. Comments: Affinity estimated based on fluorescence response data provided in Supplementary Table 4.

Sequence (): 5'dGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdApdTpdGpdGpdApdTpdTpdTpdTpdCpdCpdApdTpdCpdApdApdCpdGpdApdApdGpdTpdGpdCpdGpdTpdCpdCpdGpdTpdCpdCpdCp3' Length: 48 Molecular Weight: 14720.56 g/mole Extinction Coefficient: 446300 L/(mole·cm) GC Content: 58.33% nmoles/OD₂₆₀: 2.24 μg/OD₂₆₀: 32.98

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors. J. Am. Chem. Soc.134:1642-1647.

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Aptamer Details

Beta-Estradiol Aptamer (BES.1) (ID# 609)

Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors. J. Am. Chem. Soc.134:1642-1647.

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