Aptagen, LLC
DNA
Insulin
Protein
n/a nM (reported value)
50 mM Tris-HCl, 10 mM KCl, 100 mM NaCl
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specificity tested against Streptavidin, Avidin, and BSA.
FAM-Labeled at 3' end.
Insulin Binding Aptamer (IBA) (ID# 608)
5'dGpdGpdTpdGpdGpdTpdGpdGpdGpdGpdGpdGpdGpdGpdTpdTpdGpdGpdTpdApdGpdGpdGpdTpdGpdTpdCpdTpdTpdCp3'
30
9493.19 g/mole
288200 L/(mole·cm)
66.67%
3.47
32.94
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Pu, Y.; Zhu, Z.; Han, D.; Liu, H.; Liu, J.; Liao, J.; Zhang, K.; & Tan, W. Insulin-Binding Aptamer-Conjugated Graphene Oxide for Insulin Detection. Analyst, 2011, 136, 4138-4140
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