* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Sodium Ion Other 39.1 mM (reported value)Dulbecco’s modification of Eagle’s medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin,N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.SUBSTRATE SEQUENCE (spaces added before and after the ribonucleic A):
dCpdTpdCpdTpdApdTpdGpdTpdApdTp
rAp
dGpdGpdApdApdGpdTpdApdCpdCpdGpdCpdCpdGpdCp
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Torabi, S., Wu, P., McGhee, C., Chen, L., Hwang, K., Zheng, N., Cheng, J., & Lu, Y. (2015) In Vitro Selection of a Sodium-Specific DNAzyme and its Application in Intracellular Sensing. PNAS 112, (19):5903-5908
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
Sodium Ion
Other
39.1 mM (reported value)
Dulbecco’s modification of Eagle’s medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin,
N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
SUBSTRATE SEQUENCE (spaces added before and after the ribonucleic A):
dCpdTpdCpdTpdApdTpdGpdTpdApdTp
rAp
dGpdGpdApdApdGpdTpdApdCpdCpdGpdCpdCpdGpdCp
