Aptagen, LLC
DNA
Sodium Ion
Other
39.1 mM (reported value)
Dulbeccoƒ??s modification of Eagleƒ??s medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin,
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Tested against the following ions: Li, K, Rb, Cs, NH4, Mg, Ca, Sr, Ba, Mn, Co, Ni, Cu, Zn, Cd, Hg, Pb, In, La, Eu, Sm, Yb
SUBSTRATE SEQUENCE (spaces added before and after the ribonucleic A): dCpdTpdCpdTpdApdTpdGpdTpdApdTp rAp dGpdGpdApdApdGpdTpdApdCpdCpdGpdCpdCpdGpdCp
NaA43 (ID# 603)
5'dGpdCpdGpdGpdCpdGpdGpdTpdApdCpdCpdApdGpdGpdTpdCpdApdApdApdGpdGpdTpdGpdGpdGpdTpdGpdApdGpdGpdGpdGpdApdCpdGpdCpdCpdApdApdGpdApdGpdTpdCpdCpdCpdCpdGpdCpdGpdGpdTpdTpdApdCpdApdTpdApdGpdApdGp3'
61
19035.35 g/mole
602700 L/(mole·cm)
63.93%
1.66
31.58
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Torabi, S., Wu, P., McGhee, C., Chen, L., Hwang, K., Zheng, N., Cheng, J., & Lu, Y. (2015) In Vitro Selection of a Sodium-Specific DNAzyme and its Application in Intracellular Sensing. PNAS 112, (19):5903-5908
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