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Apta-Index™: Uranium DNAzyme (ID# 598)

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Uranium DNAzyme (ID# 598)

Uranium DNAzyme
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Uranium Other n/a nM (reported value) 0.4 M NaCl and 20 mM MES buffer solution (pH 5.5) at 20?C for 15 min. To quench: 5 uL of 0.5 M Tris base solution added then sample was vortexed to shift the pH from 5.5 to 8.0. 1.0 mL 9.6 × 10-5 M DNAzyme 1.0 mL 6.4 × 10-5 M TAMRA-modified substrate Mixed with 1.0 mL of 2.0 M NaCl and 1.0 mL of 20 mM MES buffer solution (pH 5.5). Heat at 90?C for 15 min and cooled down t Limit of Detection: 0.41 nM Uranium Substrate Sequence: TAMRA-CCTC ACTAT rA GGAAG AGATG GACGT G
dCpdApdCpdGpdTp dCpdCpdApdTpdCp dTpdCpdTpdGpdCp dApdGpdTpdCpdGp dGpdGpdTpdApdGpdTpdTpdApdApdAp dCpdCpdGpdApdCp dCpdTpdTpdCpdAp dGpdApdCpdApdTp dApdGpdTpdApdAp dGpdGpdGpdGp
54 16630.84 g/mole 524500.00 L/(mole·cm) 51.85% 1.91 31.71

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Sai Jin Xiao, Jun Zuo, Zhi Qiang Zhu, Yong Zhong Ouyang, Xing Lei Zhang, Huan Wen Chen, & Li Zhang (2015) Highly Sensitive DNAzyme Sensor for Selective Detection of Trace Uranium in Ore and Natural Water Samples. Sensors and Actuators B 210 (2015) 656-660

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