Aptagen, LLC
DNA
Glioblastoma
Cells
20 nM (reported value)
The washing buffer (WB) used contained 4.5 g/L glucose, and 5 mM MgCl2 in PBS (pH = 7.4). The binding buffer used during selection was prepared by adding yeast tRNA (0.1 mg/mL) and BSA (1 mg/mL) to washing buffer to reduce background binding.
4°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
GBM128 (ID# 589)
277
25689.73 g/mole
812600 L/(mole·cm)
49.40%
1.23
31.61
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kang D, Wang J, Zhang W, Song Y, Li X, et al. (2012) Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity. PLoS ONE 7(10): e42731. doi:10.1371/journal.pone.0042731
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