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Apta-Index™: Aptamer 25 (ID# 587)

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Aptamer 25 (ID# 587)

Aptamer 25
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Human norovirus (SMV-VLP) Other 232 nM (reported value) Phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20 Heat at 90°C for 5 min The library was amplified using a fluorescein (FAM)-labeled forward constant region primer [5'-56-FAM/-AGTATACGTATTACCTGCAGC-3'] and a biotinylated reverse constant region primer [5'-/5Bios/-GCAAGATCTCCGAGATATCG-3']
dApdGpdTpdApdTpdApdCpdGpdTpdApdTpdTpdApdCpdCpdTpdGpdCpdApdGpdCpdCpdApdTpdCpdTpdGpdTpdGpdTpdGpdApdApdGpdApdCpdTpdApdTpdApdTpdGpdGpdCpdGpdCpdTpdCpdApdCpdApdTpdApdTpdTpdTpdCpdTpdTpdTpdCpdCpdGpdApdTpdApdTpdCpdTpdCpdGpdGpdApdGpdApdTpdCpdTpdTpdGpdCp
81 24864.21 g/mole 770500.00 L/(mole·cm) 43.21% 1.30 32.27

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Escudero-Abarca, B. I. et al. "Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains." PLOS One 2014: 1-11.

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