Aptamer Details

Aptamer 25 (ID# 587)

Aptamer Chemistry: DNA Target: Human norovirus (SMV-VLP) Antigen/Target Category: Other Affinity (Kd): 232 nM (reported value) Binding Conditions/Buffer: Phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20 Binding Temp: 23°C Refolding Protocol: Heat at 90°C for 5 min Comments: The library was amplified using a fluorescein (FAM)-labeled forward constant region primer [5'-56-FAM/-AGTATACGTATTACCTGCAGC-3'] and a biotinylated reverse constant region primer [5'-/5Bios/-GCAAGATCTCCGAGATATCG-3']

Sequence (): Length: 243 Molecular Weight: 24864.21 g/mole Extinction Coefficient: 770500 L/(mole·cm) GC Content: 43.21% nmoles/OD₂₆₀: 1.30 μg/OD₂₆₀: 32.27

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Escudero-Abarca, B. I. et al. "Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains." PLOS One 2014: 1-11.

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Aptamer Details

Aptamer 25 (ID# 587)

Escudero-Abarca, B. I. et al. "Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains." PLOS One 2014: 1-11.

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