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Apta-Index™: A15 (ID# 558)

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A15 (ID# 558)

A15
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Staphylococcus aureus enterotoxin A (SEA) Protein 48.57 nM (reported value) 20 mM Tris, 100 mM NaCl, 5 mM KCl, 1 mM CaCl2 and 1 mM MgCl2 • 6H2O, pH 7.4 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. ssDNA aptamers specific for SEA were selected using a 12-round SELEX procedure. A15 was used successfully in fluorescent bioassay to detect SEA in the food sample with a detection limit of 8.7 x 10^(-3) ug/mL.
dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdApdCpdTpdTpdApdTpdGpdCpdApdTpdTpdTpdCpdCpdTpdCpdCpdCpdApdCpdGpdApdTpdCpdTpdTpdApdTpdTpdTpdGpdApdGpdApdGpdTpdGpdApdCpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp
80 24604.02 g/mole 768000.00 L/(mole·cm) 47.50% 1.30 32.04

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Huang, Y. et al. "Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A." Anal. Methods, 2014, 6, 690-697.

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