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Apta-Index™: StrepApt 2 (ID# 550)

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StrepApt 2 (ID# 550)

StrepApt 2
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Streptavidin Protein 77 nM (reported value) NaCl (100 mM), MgCl2 (2 mM), KCl (5 mM), CaCl2 (1 mM), Tris-HCl (pH 7.6, 20 mM) Heated to 95C for 8 minutes, cool on ice for 10 minutes and left at room temperature for at least 10 minutes. Ka = 3.98 x 10^4 kd = 3.07 x 10^-3 Best KD with highest Ka.
dGpdGpdGpdApdApdTpdCpdGpdCpdCpdApdCpdCpdCpdGpdApdCpdGpdCpdApdGpdGpdGpdTpdTpdTpdCpdCpdCp
29 8864.76 g/mole 267200.00 L/(mole·cm) 68.97% 3.74 33.18

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Ruigrok, Vincent J. B., et al. "Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers." ChemBioChem 13 (2012): 829-836. Print.

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