Aptamer Details

StrepApt 2 (ID# 550)

Aptamer Chemistry: DNA Target: Streptavidin Antigen/Target Category: Protein Affinity (Kd): 77 nM (reported value) Binding Conditions/Buffer: NaCl (100 mM), MgCl2 (2 mM), KCl (5 mM), CaCl2 (1 mM), Tris-HCl (pH 7.6, 20 mM) Binding Temp: °C Refolding Protocol: Heated to 95C for 8 minutes, cool on ice for 10 minutes and left at room temperature for at least 10 minutes. Comments: Ka = 3.98 x 10^4 kd = 3.07 x 10^-3 Best KD with highest Ka.

Sequence (): Length: 87 Molecular Weight: 8864.76 g/mole Extinction Coefficient: 267200 L/(mole·cm) GC Content: 68.97% nmoles/OD₂₆₀: 3.74 μg/OD₂₆₀: 33.18

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ruigrok, Vincent J. B., et al. "Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers." ChemBioChem 13 (2012): 829-836. Print.

Have your aptamer oligo synthesized ORDER NOW

Aptamer Details

StrepApt 2 (ID# 550)

Ruigrok, Vincent J. B., et al. "Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers." ChemBioChem 13 (2012): 829-836. Print.

Related Aptamer Targets

Community Feedback