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Apta-Index™: Aptamer ID 3 (ID# 543)

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Aptamer ID 3 (ID# 543)

Aptamer ID 3
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA PDGF-BB Protein 2.6 nM (reported value) 0.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2 (PBSM), pH 7.4 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. 3 rounds of positive selection at increasing selection stringencies
dTpdCpdCpdCpdApdCpdGpdCpdApdTpdTpdCpdTpdCpdCpdApdCpdApdTpdCpdApdGpdTpdTpdGpdApdApdTpdGpdGpdTpdGpdTpdGpdGpdTpdCpdApdCpdTpdTpdCpdCpdApdGpdTpdCpdCpdCpdGpdCpdApdGpdGpdGpdCpdApdCpdApdCpdCpdCpdTpdTpdTpdCpdTpdGpdTpdCpdCpdTpdTpdCpdCpdGpdTpdCpdApdCp
80 24315.72 g/mole 715600.00 L/(mole·cm) 56.25% 1.40 33.98

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Cho et al. "Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing." PNAS 2010, 1-6.

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