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Apta-Index™: Aptamer ID 2 (ID# 542)

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Aptamer ID 2 (ID# 542)

Aptamer ID 2
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA PDGF-BB Protein 2.5 nM (reported value) 0.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2 (PBSM), pH 7.4 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. 3 rounds of positive selection at increasing selection stringencies
dTpdCpdCpdCpdApdCpdGpdCpdApdTpdTpdCpdTpdCpdCpdApdCpdApdTpdCpdGpdApdTpdApdCpdTpdGpdApdGpdCpdApdTpdCpdGpdTpdApdCpdApdTpdGpdApdTpdCpdCpdCpdGpdCpdApdApdCpdGpdGpdGpdCpdApdGpdTpdApdTpdTpdCpdCpdTpdTpdTpdCpdTpdGpdTpdCpdCpdTpdTpdCpdCpdGpdTpdCpdApdCp
80 24307.75 g/mole 728600.00 L/(mole·cm) 52.50% 1.37 33.36

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Cho et al. "Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing." PNAS 2010, 1-6.

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