* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA gastric cancer cell-line HGC-27 Cells 16.49 nM (reported value)4.5 g/l glucose, 5 mM MgCl2,2 mg/ml BSA and 0.2 mg/ml yeast tRNA dissolved in PBS bufferN/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.HGC-27 was used as target (positive cell line) and GES-1 as counter selection (negative cell line)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhang, X. et al. "A cell-based single-stranded DNA aptamer specifically targets gastric cancer." The International Journal of Biochemistry & Cell Biology 2014, 46: 1-8.
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
gastric cancer cell-line HGC-27
Cells
16.49 nM (reported value)
4.5 g/l glucose, 5 mM MgCl2,2 mg/ml BSA and 0.2 mg/ml yeast tRNA dissolved in PBS buffer
N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
HGC-27 was used as target (positive cell line) and GES-1 as counter selection (negative cell line)
