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Apta-Index™: ACG03 (ID# 532)

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ACG03 (ID# 532)

ACG03
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA gastric cancer cell-line HGC-27 Cells 16.49 nM (reported value) 4.5 g/l glucose, 5 mM MgCl2,2 mg/ml BSA and 0.2 mg/ml yeast tRNA dissolved in PBS buffer N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. HGC-27 was used as target (positive cell line) and GES-1 as counter selection (negative cell line)
5'-dApdCpdGpdCpdTpdCpdGpdGpdApdTpdGpdCpdCpdApdCpdTpdApdCpdApdGpdGpdGpdGpdGpdGpdTpdGpdGpdTpdCpdCpdTpdGpdApdGpdGpdGpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdTpdTpdTpdGpdGpdTpdTpdTpdCpdCpdTpdCpdApdTpdGpdGpdApdCpdGpdTpdGpdCpdTpdGpdGpdTpdGpdApdCp-3'
78 24321.75 g/mole 730600.00 L/(mole·cm) 60.26% 1.37 33.29

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Zhang, X. et al. "A cell-based single-stranded DNA aptamer specifically targets gastric cancer." The International Journal of Biochemistry & Cell Biology 2014, 46: 1-8.

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