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Apta-Index™: HIV-1 Reverse Transcriptase (4.3) (ID# 53)

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HIV-1 Reverse Transcriptase (4.3) (ID# 53)

HIV-1 Reverse Transcriptase (4.3)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA HIV-1 RT Protein 190 pM (reported value) Binding buffer: 10 mM tris, 50 mM NaCl, and 1 mM EDTA (pH 7.5) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdGpdCpdApdGpdGpdTpdTpdTpdCpdGpdApdCpdGpdTpdApdCpdApdApdTpdGpdCpdTpdApdTpdGpdGpdApdGpdGpdCpdTpdTpdTpdApdTpdGpdApdTpdCpdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
80 24799.14 g/mole 780100.00 L/(mole·cm) 51.25% 1.28 31.79

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Mosing et al. "Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase." Analytical Chemistry, 77 (2005): 6107-6112.

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